HPLC法测定华法林片的含量
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【摘要】 目的 建立HPLC法测华法林片的含量。方法 采用Diamonsil C18(4.6 mm×250 mm,5 μm)为分离柱；流动相：乙腈-水-冰醋酸(70∶30∶1)；流速：1 ml/min；检测波长308 nm，以峰面积计算。结果 线性范围：0.8~5.6 μg/ml，r=0.999 6。平均回收率为100.64%，RSD=1.11%,n=15。结论 本法简便，快捷，结果准确，重现性好，可作为华法林片质量控制的有效方法。
【关键词】High performance liguid chromatography；华法林；含量测定
Determination of warfarin in tablets by HPLC
HUANG Bi-ying,YAO Qiu-yan,LI Min-wei,et al.Dept of Pharmacy,the First Affiliated Hospital of Sun Yat-sen University,Guangdong,Guangzhou 510080，China
【Abstract】 Objective To establish a HPLC method to measure the content of warfarin tablets.Methods A Diamonsil C18column(4.6 mm×250 mm,5 μm)was used.The mobile phase was a mixture of 70%acetonitrile,30%water and 1%glacial acetic acid,and the flow rate was 1.0ml/min.The detection wavelength was at 308 nm.Results The linear calibration curves were obtained over the range 0.8 μg/mlto 5.6 μg/ml of warfarin,r=0.999 6.The recovery of the assay was 100.64%(n=15).The coefficients of variation was 1.11%.Conclusion The method appeared to be simple,convenient and precise,and can be used for control of warfarinin tablets. ......
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