皮肤鳞状细胞癌及Bowen病中PGP9.5和CyclinD1的表达及临床意义(1)
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[摘要]目的：探讨 PGP9.5和CyclinD1在皮肤鳞状细胞癌(CSCC)、Bowen病中的表达及临床意义。方法：用免疫组织化学SP法检测22例CSCC，31例Bowen病及10例正常皮肤组织中PGP9.5和CyclinD1的表达情况。结果：PGP9.5与CyclinD1在CSCC的表达明显高于正常皮肤组(P=0.006，P=0.00057，<0.05)和Bowen病组(P<0.05)，PGP9.5与CyclinD1在低分化鳞癌的表达明显强于高分化鳞癌的表达(P<0.05)。在CSCC、Bowen病中，PGP9.5与CyclinD1的阳性表达存在相关性。结论：PGP9.5、CyclinD1的高表达和鳞癌细胞分化程度有密切关系。
[关键词]皮肤鳞状细胞癌(CSCC)；Bowen病；PGP9.5；CyclinD1；免疫组织化学
[中图分类号]Q813.1 [文献标识码]A [文章编号]1008-6455(2012)03-0424-03
Expression and Significance of PGP9.5 and CyclinD1 in Human Skin Squamous Cell Carcinoma and Bowen's Disease
NIE Jian-jun1,2,ZENG wei-hui1,GENG song-mei1,XU-lei2
(1.Department of Dermatology,The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710004,Shaanxi,China; 2.Department of Dermatology of The Second Hospital of Chengdu )
Abstract: Objective To study the expression of PGP9.5and CyclinD1 in Cutaneous squamous cell carcinoma and Bowen's disease and exploring the relationship between them and to confirm their role in development of the tumor. Methods Immunohistochemical S-P method was used to examine the expressions of PGP9.5 and CyclinD1 in 22 cases of CSCC, 31cases of Bowen's disease, and 10 cases of normal skin. Results The expression of PGP9.5 and CyclinD1 was significantly higher in CSCC than in normal tissue and Bowen's disease (P=0.006,P=0.00057<0.05). The expression of PGP9.5 and CyclinD1 was significantly stronger in poorly differentiated CSCC than in well differentiated CSCC(P<0.05). The positive expression of PGP9.5 and CyclinD1 are positive correlations in CSCC and Bowen's disease. Conclusion The co-expression of PGP9.5 and CyclinD1 is related with carcinogenesis and m alignant progression of CSCC and Bowen's disease.
Key words:Cutaneous Squamous cell carcinoma(CSCC); Bowen's disease;PGP9.5;CyclinD1; immunohistochemistry
蛋白基因产物9.5(protein gene product,PGP9.5)属于泛素水解酶家族，广泛分布于神经元分化的各个阶段，为神经内分泌肿瘤的标志物[1-2]。近年发现PGP9.5在肺、食管及间充质等的肿瘤有高表达[3-6]。PGP9.5通过泛素-蛋白酶体途径降解细胞核内蛋白及细胞周期调节蛋白。细胞周期蛋白D1(CyclinD1)属于细胞周期调节因子，在肿瘤的发生中起很重要的作用。有关PGP9.5介导的泛素-蛋白酶体途径在CyclinD1参与的调节细胞周期蛋白的机制中发挥的作用，及PGP9.5和CyclinD1在肿瘤的发生发展所扮演的角色受到了学者们的关注。笔者应用免疫组化SP法检测皮肤鳞状细胞癌、Bowen病及正常皮肤组织中PGP9.5及CyclinD1的表达情况，现报道如下。
1 材料和方法
1.1 材料收集：2006年1月至2010年3月西安交通大学医学院第二附属医院皮肤科皮肤鳞状细胞癌蜡块22例，其中男10例，女12例，年龄56～83岁Bowen病31例 ......
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