基于古代本草记载的不同产地艾叶中棕矢车菊素和异泽兰黄素的含量研究(1)
[摘要]为了评价“海艾”、“蕲艾”、“北艾”、“祁艾”和南阳艾叶品质，利用高效液相色谱法同时测定艾叶中棕矢车菊素和异泽兰黄素的含量。采用Agilent Eclipse XDBC18色谱柱(46 mm×250 mm， 5 μm)，流动相为乙腈02%磷酸溶液(35∶65)，流速 10 mL·min-1，检测波长350 nm，柱温 25 ℃。棕矢车菊素和异泽兰黄素分别在0003～0126 g·L-1 (r=0999 9)，0005～0200 g·L-1 (r=0999 9)呈良好的线性关系，平均回收率(n=6) 分别为9914% (RSD 12%)，9940% (RSD 073%)，说明该方法准确可靠、重复性好，是用于艾叶的质量控制的有效方法。结果表明不同产地艾叶中棕矢车菊素含量差异较小，不同产地艾叶中异泽兰黄素的含量差异较大，淮河以南的浙江宁波、湖北蕲春、湖北襄阳、河南南阳的艾叶和淮河以北汤阴艾叶、安国艾叶中异泽兰黄素含量相对较高。
[关键词]艾葉； 棕矢车菊素； 异泽兰黄素； 含量研究
, 百拇医药
Quantative analysis of eupatilin and jaceosidin in folium of Artemisia argyi from
different areas in China by RPHPLC based on ancient medicine books
HUANG Xianzhang1，2，3， KANG Liping1， GAO Li3， ZHANG Yuan1， GUO Lanping1， HUANG Luqi1*
(1. State Key Laboratory Breeding Base of Daodi Herbs， National Resource Center for Chinese Materia Medica，China Academy of Chinese Medical Sciences， Beijing 100700， China；
, 百拇医药
2. Postdoctoral Research Station， China Academy of Chinese Medical Sciences， Beijing 100700， China；
3. Henan Province Key Laboratory of Zhang Zhongjing Formulae and Herbs for Immunoregulation，Nanyang Institute of Technology， Nanyang 473004， China)
[Abstract]In order to evaluate the quality of Artemisia argyi from Qichun， Ningbo， Anguo and Nanyang， the contents of eupatilin and jaceosidin were determined by RPHPLC The determination was performed on Agilent Eclipse XDBC18 (46 mm×250 mm， 5 μm) with mobile phase consisted of acetonitrile02% phosphoric acid(35∶65) at the flow rate 10 mL·min-1 The detection wavelength was 350 nm and the column temperature was 25 ℃ The results showed that the amount of eupatilin and jaceosidin had a clear linear relationship in the range of 00030126 g·L-1 (r=0999 9) and 00050200 g·L-1 (r=0999 9)， and the average recovery rates for them were 9914% (n=6， RSD 12%) and 9940% (n=6， RSD=073%)， respectively The results showed that RPHPLC can be used for the quantification of eupatilin and jaceosidin in the folium of A argyi With this method， we found there was no significant difference of jaceosidin content within all the samples collected， but the content of eupatilin was significantly higher in samples from Qichun， Ningbo， Xiangyang and Nanyang， located in the south of Huaihe River compared with these from other areas
, 百拇医药
[Key words]folium of Artemisia argyi； jaceosidin； eupatilin； quantative analysis
艾叶为菊科植物艾Artemisia argyi Levl et Vant 的干燥叶，具有温经止血、散寒止痛的作用[1]，为我国常用中药材。我国现存第一部医方书《五十二病方》[2]和《黄帝内经》中均有使用艾叶治疗疾病的记载，但历代本草对艾叶道地产地的记载不尽相同。宋代苏颂《本草图经》载：“艾叶、旧不著所出州土，但云生田野，今处处有之，以复道(及四明)者为佳”[3] 。明代刘文泰等撰写的《本草品汇精要》中有艾叶“道地：蕲州、明州”的记载[4]。明代李时珍则对艾叶的道地产地作了详细描述：“(艾叶)宋时以汤阴复道者为佳，四明者图形。近代惟汤阴者谓之北艾，四明者谓之海艾”[5]。《中国道地药材》把产自河北安国(古称祁州)的艾叶称为“祁艾”，并提出了“河北安国的祁艾和湖北蕲春的蕲艾道地特性有待比较”的观点[6]。时至今日，历代本草文献明确提出的艾叶道地产地有“四明”、“复道”、“蕲州”、“祁州”等地，即现在的浙江宁波、河南汤阴、湖北蕲春和河北安国境内，且宁波艾叶被称为“海艾”，汤阴艾叶被称为“北艾”，安国艾叶被称为“祁艾”。, 百拇医药(黄显章康利平高丽张元郭兰萍黄璐琦)
[关键词]艾葉； 棕矢车菊素； 异泽兰黄素； 含量研究
, 百拇医药
Quantative analysis of eupatilin and jaceosidin in folium of Artemisia argyi from
different areas in China by RPHPLC based on ancient medicine books
HUANG Xianzhang1，2，3， KANG Liping1， GAO Li3， ZHANG Yuan1， GUO Lanping1， HUANG Luqi1*
(1. State Key Laboratory Breeding Base of Daodi Herbs， National Resource Center for Chinese Materia Medica，China Academy of Chinese Medical Sciences， Beijing 100700， China；
, 百拇医药
2. Postdoctoral Research Station， China Academy of Chinese Medical Sciences， Beijing 100700， China；
3. Henan Province Key Laboratory of Zhang Zhongjing Formulae and Herbs for Immunoregulation，Nanyang Institute of Technology， Nanyang 473004， China)
[Abstract]In order to evaluate the quality of Artemisia argyi from Qichun， Ningbo， Anguo and Nanyang， the contents of eupatilin and jaceosidin were determined by RPHPLC The determination was performed on Agilent Eclipse XDBC18 (46 mm×250 mm， 5 μm) with mobile phase consisted of acetonitrile02% phosphoric acid(35∶65) at the flow rate 10 mL·min-1 The detection wavelength was 350 nm and the column temperature was 25 ℃ The results showed that the amount of eupatilin and jaceosidin had a clear linear relationship in the range of 00030126 g·L-1 (r=0999 9) and 00050200 g·L-1 (r=0999 9)， and the average recovery rates for them were 9914% (n=6， RSD 12%) and 9940% (n=6， RSD=073%)， respectively The results showed that RPHPLC can be used for the quantification of eupatilin and jaceosidin in the folium of A argyi With this method， we found there was no significant difference of jaceosidin content within all the samples collected， but the content of eupatilin was significantly higher in samples from Qichun， Ningbo， Xiangyang and Nanyang， located in the south of Huaihe River compared with these from other areas
, 百拇医药
[Key words]folium of Artemisia argyi； jaceosidin； eupatilin； quantative analysis
艾叶为菊科植物艾Artemisia argyi Levl et Vant 的干燥叶，具有温经止血、散寒止痛的作用[1]，为我国常用中药材。我国现存第一部医方书《五十二病方》[2]和《黄帝内经》中均有使用艾叶治疗疾病的记载，但历代本草对艾叶道地产地的记载不尽相同。宋代苏颂《本草图经》载：“艾叶、旧不著所出州土，但云生田野，今处处有之，以复道(及四明)者为佳”[3] 。明代刘文泰等撰写的《本草品汇精要》中有艾叶“道地：蕲州、明州”的记载[4]。明代李时珍则对艾叶的道地产地作了详细描述：“(艾叶)宋时以汤阴复道者为佳，四明者图形。近代惟汤阴者谓之北艾，四明者谓之海艾”[5]。《中国道地药材》把产自河北安国(古称祁州)的艾叶称为“祁艾”，并提出了“河北安国的祁艾和湖北蕲春的蕲艾道地特性有待比较”的观点[6]。时至今日，历代本草文献明确提出的艾叶道地产地有“四明”、“复道”、“蕲州”、“祁州”等地，即现在的浙江宁波、河南汤阴、湖北蕲春和河北安国境内，且宁波艾叶被称为“海艾”，汤阴艾叶被称为“北艾”，安国艾叶被称为“祁艾”。, 百拇医药(黄显章康利平高丽张元郭兰萍黄璐琦)