贵州天名精中倍半萜内酯类的化学成分研究(1)
[摘要] 利用各种色谱技术从贵州天名精的丙酮提取部位分离得18个倍半萜内酯化合物。通过全面的光谱数据鉴定了它们的结构，涉及2个卡拉布烷型倍半萜天名精内酯酮(1)，4R-天名精内酯醇(2)； 3个桉烷型倍半萜大叶土木香内酯(3)，3-epi-isotelekin(4)，1α-hydroxypinatifidin(5)； 8个愈创木烷型倍半萜[4β，10α-dihydroxy-5α(H)-1，11(13)-guaidien-8α，12-olide(6)，8-epi-helenium lactone(7)，4-epi-isoinuviscolide(8)，9β，10β-epoxy-4α-hydroxy-1β-H，11α-H-guaian-8α，12-olide(9)，4α，10α-dihydroxy-1β(H)，5β(H)-guaian-11(13)-en-8α，12-olide(10)，4α-hydroxy-9β，10β-epoxy-11β-H，5α-H-guaian-11(13)-en-8α，12-olide(11)，4α-hydroxy-1β，5α， 11α-H-guaian-9(10)-en-8α，12-olide(12)，inuviscolide(13)]，1个伪愈创木烷型倍半萜[(+)-confertin(14)； 3个吉马烷型倍半萜madolin B(15)，carabrolactone A(16)，11(13)-去氢腋生依瓦菊素(17)； 及1个苍耳烷型倍半萜tomentosin(18)，其中，化合物1的绝对构型用X-射线单晶衍射法确认，而化合物2的C-4位手性中心的绝对构型用改良mosher法确定。化合物2～5，7，9～15均为首次从本种分离得到，而其中化合物4～5，7，12～15又为首次从本属植物分离得到。另外，采用大鼠嗜铬细胞瘤细胞(PC12细胞)为研究模型，在细胞形态观察和细胞分化率统计学分析的基础上评价了化合物1～3，6和17的神经营养活性，但研究结果显示它们均无活性。
[关键词]贵州天名精； 倍半萜内酯； X-射线单晶衍射法； 改良mosher法； 神经营养
[Abstract]By using various chromatographic techniques，18 sesquiterpene lactones were isolated from the acetone extract of Carpesium faberi. Their structures were identified on the basis of comprehensive spectroscopic data， involving 2 carabrane sesquiterpenoids[carabrone(1)， 4R-carabrol(2)]， 3 eudesmane sesquiterpenoids[granilin(3)， 3-epi-isotelekin(4)， 1α-hydroxypinatifidin(5)]， 8 guaiane sesquiterpenoids[4β，10α-dihydroxy-5α(H)-1，11(13)-guaidien-8α，12-olide(6)， 8-epi-helenium lactone(7)， 4-epi-isoinuviscolide(8)， 9β，10β-epoxy-4α-hydroxy-1β-H，11α-H-guaian-8α，12-olide(9)， 4α，10α-dihydroxy-1β(H)，5β(H)-guaian-11-(13)-en-8α，12-olide(10)， 4α-hydroxy-9β，10β-epoxy-11β-H，5α-H-guaian-11(13)-en-8α，12-olide(11)， 4α-hydroxy-1β，5α，11α-H-guaian-9(10)-en-8α，12-olide(12)， inuviscolide(13)]， 1 pseudoguaiane sesquiterpenoid[(+)-confertin(14)]， 3 germacrane sesquiterpenoids[madolin B(15)， carabrolactone A(16)，11(13)-dehydroivaxillin(17)]， 1 xanthane sesquiterpenoid[tomentosin(18)]. Furthermore， the absolute configuration of 1 was confirmed by Cu-Kα X-ray crystallographic analysis，and the R-configuration of the chiral center at C-4 in 2 was established by the modified Mosher′s method.The compounds 2-5， 7 and 9-15 were isolated from this plant for the first time， and compounds 4-5， 7， and 12-15 were isolated from this genus for the first time.In addition， the neurotrophic activity of compounds 1-3， 6 and 17 were evaluated by morphological observation and statistical analysis of cells differentiation， using rat pheochromocytoma(PC12)cells as a model system. However， all compounds were inactive. (杨勇勋)
[关键词]贵州天名精； 倍半萜内酯； X-射线单晶衍射法； 改良mosher法； 神经营养
[Abstract]By using various chromatographic techniques，18 sesquiterpene lactones were isolated from the acetone extract of Carpesium faberi. Their structures were identified on the basis of comprehensive spectroscopic data， involving 2 carabrane sesquiterpenoids[carabrone(1)， 4R-carabrol(2)]， 3 eudesmane sesquiterpenoids[granilin(3)， 3-epi-isotelekin(4)， 1α-hydroxypinatifidin(5)]， 8 guaiane sesquiterpenoids[4β，10α-dihydroxy-5α(H)-1，11(13)-guaidien-8α，12-olide(6)， 8-epi-helenium lactone(7)， 4-epi-isoinuviscolide(8)， 9β，10β-epoxy-4α-hydroxy-1β-H，11α-H-guaian-8α，12-olide(9)， 4α，10α-dihydroxy-1β(H)，5β(H)-guaian-11-(13)-en-8α，12-olide(10)， 4α-hydroxy-9β，10β-epoxy-11β-H，5α-H-guaian-11(13)-en-8α，12-olide(11)， 4α-hydroxy-1β，5α，11α-H-guaian-9(10)-en-8α，12-olide(12)， inuviscolide(13)]， 1 pseudoguaiane sesquiterpenoid[(+)-confertin(14)]， 3 germacrane sesquiterpenoids[madolin B(15)， carabrolactone A(16)，11(13)-dehydroivaxillin(17)]， 1 xanthane sesquiterpenoid[tomentosin(18)]. Furthermore， the absolute configuration of 1 was confirmed by Cu-Kα X-ray crystallographic analysis，and the R-configuration of the chiral center at C-4 in 2 was established by the modified Mosher′s method.The compounds 2-5， 7 and 9-15 were isolated from this plant for the first time， and compounds 4-5， 7， and 12-15 were isolated from this genus for the first time.In addition， the neurotrophic activity of compounds 1-3， 6 and 17 were evaluated by morphological observation and statistical analysis of cells differentiation， using rat pheochromocytoma(PC12)cells as a model system. However， all compounds were inactive. (杨勇勋)