丹参通络胶囊对心梗大鼠心肌的保护作用和血流动力学的影响(1)
[摘要]研究丹参通络胶囊对心梗模型大鼠心脏的保护作用。SD大鼠高脂喂养1个月，根据心脏超声左室射血分数(EF)随机分成假手术组和模型组，造模采用冠状动脉结扎复制心梗模型，术后分为5组：模型组、阳性对照组(卡托普利)、丹参通络低、中、高剂量组。给药4周检测血流动力学、心脏超声及电镜观察心肌超微结构变化。结果显示：①丹参通络胶囊各剂量组对左心室舒张末压(LVEDP)有明显的降低作用(P<0.01)，升高了左心室内压最大上升速率(+dp/dtmax)，降低左心室内压下降速率(-dp/dtmax)，且中、高剂量组有统计学意义(P<0.05);②B超结果显示丹参通络胶囊各剂量组升高EF和左室短轴缩短率(FS)(P<0.01);③中剂量组心梗指数明显降低(P<0.01)，心脏超微结构可见缺血心肌肌丝肌节损伤、断裂，丹参通络各组减少心肌损伤，缩小心梗面积。因此可初步推断丹参通络胶囊能够改善血流动力学的功能，对缺血心肌有保护作用。
[关键词]丹参通络胶囊;心梗;左室射血分数;血流动力学;大鼠
, http://www.100md.com
[收稿日期]2014-06-11
[基金项目]国家“重大新药创制”科技重大专项(2012ZX09102201-006，2013ZX09301307)
[通信作者]*黄世敬，博士，研究员，主任医师，硕士生导师，研究方向为中医脑病，E-mail：gamhsj@126.com
[作者简介]张颖，博士，研究方向为中医脑病，E-mail：zhangying1179@163.comStudy on protective and haemodynamic effects of Danshen Tongluo
capsule on rats with myocardial infarction
ZHANG Ying， HUANG Shi-jing*， WANG Yan-yun， PAN Ju-hua， LI Duo-jiao， ZHENG Jun， CHEN Chao
, 百拇医药
( Guang′anmen Hospital， China Academy of Medical Sciences， Beijing 100053， China)
[Abstract]To study the protective effect of Danshen Tongluo capsule on rat hearts in the myocardial infarction (MI) model. After being fed with high fat diets for one month， the SD rats were randomly divided into the sham group and the model group according to the left ventricular ejection fraction (EF). The MI model group was duplicated by ligating coronary artery and then divided into five groups： the model group， the positive control group (model+captopril)， the small dose group (model+Danshen Tongluo)， the medium dose group (model + Danshen Tongluo) and the high dose group (model+Danshen Tongluo). Four weeks later， changes in myocardium ultra-structure were observed by hemodynamics， cardiac ultrasound and electron microscope. The results showed： ① All doses of Danshen Tongluo capsule could significantly reduce the left ventricular end diastolic pressure (LVEDP) (P<0.01)， increase the maximum internal pressure of the left ventricle (+dp/dtmax)， and lower the drop rate of left ventricular pressure (-dp/dtmax)， with statistical significances in medium and high dose groups; ② The B ultrasound results showed increase in EF and left ventricular shortening fraction (FS) in all dose groups of Danshen Tongluo capsule; ③ The medium dose group showed significant decrease in myocardial infarction index (P<0.01) and injured and fractured myofilament and sarcomere of ischemic myocardium in myocardial ultra-structure; All of Danshen Tongluo capsule-treated groups revealed reduction in myocardial injury and myocardial infraction area. The study preliminarily proves that Danshen Tongluo capsule can improve hemodynamic function， and has a protective effect on myocardial ischemia., 百拇医药(张颖 黄世敬 王彦云 潘菊华 李多娇 郑军 陈朝)
[关键词]丹参通络胶囊;心梗;左室射血分数;血流动力学;大鼠
, http://www.100md.com
[收稿日期]2014-06-11
[基金项目]国家“重大新药创制”科技重大专项(2012ZX09102201-006，2013ZX09301307)
[通信作者]*黄世敬，博士，研究员，主任医师，硕士生导师，研究方向为中医脑病，E-mail：gamhsj@126.com
[作者简介]张颖，博士，研究方向为中医脑病，E-mail：zhangying1179@163.comStudy on protective and haemodynamic effects of Danshen Tongluo
capsule on rats with myocardial infarction
ZHANG Ying， HUANG Shi-jing*， WANG Yan-yun， PAN Ju-hua， LI Duo-jiao， ZHENG Jun， CHEN Chao
, 百拇医药
( Guang′anmen Hospital， China Academy of Medical Sciences， Beijing 100053， China)
[Abstract]To study the protective effect of Danshen Tongluo capsule on rat hearts in the myocardial infarction (MI) model. After being fed with high fat diets for one month， the SD rats were randomly divided into the sham group and the model group according to the left ventricular ejection fraction (EF). The MI model group was duplicated by ligating coronary artery and then divided into five groups： the model group， the positive control group (model+captopril)， the small dose group (model+Danshen Tongluo)， the medium dose group (model + Danshen Tongluo) and the high dose group (model+Danshen Tongluo). Four weeks later， changes in myocardium ultra-structure were observed by hemodynamics， cardiac ultrasound and electron microscope. The results showed： ① All doses of Danshen Tongluo capsule could significantly reduce the left ventricular end diastolic pressure (LVEDP) (P<0.01)， increase the maximum internal pressure of the left ventricle (+dp/dtmax)， and lower the drop rate of left ventricular pressure (-dp/dtmax)， with statistical significances in medium and high dose groups; ② The B ultrasound results showed increase in EF and left ventricular shortening fraction (FS) in all dose groups of Danshen Tongluo capsule; ③ The medium dose group showed significant decrease in myocardial infarction index (P<0.01) and injured and fractured myofilament and sarcomere of ischemic myocardium in myocardial ultra-structure; All of Danshen Tongluo capsule-treated groups revealed reduction in myocardial injury and myocardial infraction area. The study preliminarily proves that Danshen Tongluo capsule can improve hemodynamic function， and has a protective effect on myocardial ischemia., 百拇医药(张颖 黄世敬 王彦云 潘菊华 李多娇 郑军 陈朝)