黄连解毒汤乙酸乙酯提取物对光滑念珠菌黏附作用的影响(1)
[摘要]目的：探讨黄连解毒汤乙酸乙酯提取物(ethyl acetate extract of Huanglian Jiedu decoction，EAHD)对光滑念珠菌Candida glabrata黏附作用的影响。方法：采用二倍稀释法测定黄连解毒汤乙酸乙酯提取物对光滑念珠菌的最低抑菌浓度(minimum inhibitory concentration，MIC);采用XTT还原法评价EAHD对光滑念珠菌黏附作用的影响;利用倒置显微镜、扫描电子显微镜与荧光显微镜观察EAHD对光滑念珠菌早期黏附形态学影响;采用半定量与实时荧光定量PCR检测其黏附相关基因EPA1，EPA6和EPA7的表达量。结果：EAHD对光滑念珠菌的MIC为320 mg·L-1，阳性对照药氟康唑对光滑念珠菌的MIC为1 mg·L-1;EAHD对光滑念珠菌2，4 h的黏附的干预作用较明显，其中对4 h效果优于2 h;PCR结果显示EAHD可显著抑制EPA1，EPA6和EPA7的表达。结论：EAHD可能通过抑制光滑念珠菌部分黏附基因的表达而抑制其早期黏附。
, http://www.100md.com
[关键词]黄连解毒汤乙酸乙酯提取物(EAHD);光滑念珠菌;黏附
[收稿日期]2014-07-01
[基金项目]国家自然科学基金项目(81073127);安徽省自然科学基金项目(1408085MH165，1508085MH163)
[通信作者]*汪长中，教授，硕士生导师，E-mail： ahwcz63@sina.com
[作者简介]汪天明，讲师， E-mail：wtm1818@163.comAnti-attachment effect of ethyl acetate extract of Huanglian Jiedu
decoction on Candida glabrata
, http://www.100md.com
WANG Tian-ming2， ZHANG Meng-xiang1， SHI Gao-xiang1， YAN Yuan-yuan1， SHAO Jing1， XIA Dan1， WANG Chang-zhong1*
(1. School of Integrated Traditional and Western Medicine， Anhui University of Chinese Medicine， Hefei 230038， China;
2. Institute of Integrated Traditional and Western Medicine， Anhui Academy of Chinese Medicine， Hefei 230038， China)
[Abstract]Objective： To investigate anti-attachment effect of ethyl acetate extract of Huanglian Jiedu decoction(EAHD)on Candida glabrata. Method： Serial 2-fold dilution assay was used to determine the minimum inhibitory concentrations MICs of EAHD to C. glabrata. XTT assay was used to evaluate the effect of EAHD against adhesion of C. glabrata. Inverted microscope， scanning electron microscope (SEM) and fluorescein diacetate (FDA) staining were applied to observe the morphological changes of C. glabrata in adhesion. PCR was adopted to inspect the expression of attachment-related genes such as EPA1，EPA6 and EPA7. Result： The MIC of EAHD and fluconazole to C. glabrata were 320 mg·L-1 and 1 mg·L-1 respectively. The total cells including budding cells decreased in a dose-dependent manner following EAHD treatment. The expressions of EPA1， EPA6 and EPA7 were downregulated dramatically after EAHD treatment. Conclusion： EAHD could effectively inhibit adherence of C. glabrata.
, http://www.100md.com
[Key words]ethyl acetate extract of Huanglian Jiedu decoction(EAHD); Candida glabrata; adhesion
doi：10.4268/cjcmm20150327
念珠菌Candida spp.是一类普遍存在于人类口腔、胃肠道以及外生殖道内的真菌，通常在黏膜上生长，也可侵入血液系统，造成一系列严重感染。近年来，随着真菌感染的病原谱发生改变，光滑念珠菌检出率正不断上升，其易对唑类甚至棘白菌素类产生耐药性，成为仅次于白念珠菌的重要致病性真菌[1-3]。黏附是光滑念珠菌形成感染的第一步[4]。不能牢固定植于宿主器官或组织表面的真菌，容易被血液及其他体液冲刷而不能形成感染，故能否抑制黏附是评判一个药物治疗光滑念珠菌感染的重要指标之一。, http://www.100md.com(汪天明 张梦翔 施高翔 严园园 邵菁 夏丹 汪长中)
, http://www.100md.com
[关键词]黄连解毒汤乙酸乙酯提取物(EAHD);光滑念珠菌;黏附
[收稿日期]2014-07-01
[基金项目]国家自然科学基金项目(81073127);安徽省自然科学基金项目(1408085MH165，1508085MH163)
[通信作者]*汪长中，教授，硕士生导师，E-mail： ahwcz63@sina.com
[作者简介]汪天明，讲师， E-mail：wtm1818@163.comAnti-attachment effect of ethyl acetate extract of Huanglian Jiedu
decoction on Candida glabrata
, http://www.100md.com
WANG Tian-ming2， ZHANG Meng-xiang1， SHI Gao-xiang1， YAN Yuan-yuan1， SHAO Jing1， XIA Dan1， WANG Chang-zhong1*
(1. School of Integrated Traditional and Western Medicine， Anhui University of Chinese Medicine， Hefei 230038， China;
2. Institute of Integrated Traditional and Western Medicine， Anhui Academy of Chinese Medicine， Hefei 230038， China)
[Abstract]Objective： To investigate anti-attachment effect of ethyl acetate extract of Huanglian Jiedu decoction(EAHD)on Candida glabrata. Method： Serial 2-fold dilution assay was used to determine the minimum inhibitory concentrations MICs of EAHD to C. glabrata. XTT assay was used to evaluate the effect of EAHD against adhesion of C. glabrata. Inverted microscope， scanning electron microscope (SEM) and fluorescein diacetate (FDA) staining were applied to observe the morphological changes of C. glabrata in adhesion. PCR was adopted to inspect the expression of attachment-related genes such as EPA1，EPA6 and EPA7. Result： The MIC of EAHD and fluconazole to C. glabrata were 320 mg·L-1 and 1 mg·L-1 respectively. The total cells including budding cells decreased in a dose-dependent manner following EAHD treatment. The expressions of EPA1， EPA6 and EPA7 were downregulated dramatically after EAHD treatment. Conclusion： EAHD could effectively inhibit adherence of C. glabrata.
, http://www.100md.com
[Key words]ethyl acetate extract of Huanglian Jiedu decoction(EAHD); Candida glabrata; adhesion
doi：10.4268/cjcmm20150327
念珠菌Candida spp.是一类普遍存在于人类口腔、胃肠道以及外生殖道内的真菌，通常在黏膜上生长，也可侵入血液系统，造成一系列严重感染。近年来，随着真菌感染的病原谱发生改变，光滑念珠菌检出率正不断上升，其易对唑类甚至棘白菌素类产生耐药性，成为仅次于白念珠菌的重要致病性真菌[1-3]。黏附是光滑念珠菌形成感染的第一步[4]。不能牢固定植于宿主器官或组织表面的真菌，容易被血液及其他体液冲刷而不能形成感染，故能否抑制黏附是评判一个药物治疗光滑念珠菌感染的重要指标之一。, http://www.100md.com(汪天明 张梦翔 施高翔 严园园 邵菁 夏丹 汪长中)