博落回的生物碱成分及细胞毒活性研究(1)
[摘要]目的：研究博落回Macleaya cordata的生物碱成分及其抗肿瘤活性。方法：采用乙醇提取和液液萃取等方法提取，硅胶，反相ODS，Sephadex LH-20柱色谱，HPLC等分离技术分离其生物碱成分，利用MS，NMR等光谱技术鉴定化合物结构，采用MTT法测定化合物对MCF-7及SF-268肿瘤细胞株的细胞毒活性。结果： 从博落回中分离鉴定了12种生物碱，分别为maclekarpine E(1)，6-丙酮基二氢白屈菜红碱(2)，岩黄连灵碱(3)，6-丙酮基二氢血根碱(4)，O-methylzanthoxyline(5)，6-甲氧基二氢血根碱(6)，spallidamine(7)，6-羟基二氢白屈菜红碱(8)，arnotianamida(9)，二氢血根碱(10)，原阿片碱(11)，别隐品碱(12)。结论：化合物1，3，7～9为首次从该植物中分离得到，化合物5为新天然产物。药理实验表明， 化合物6对MCF-7及SF-268细胞株具有显著的细胞毒活性，IC50值分别为0.61，0.54 μmol·L-1。
, 百拇医药
[关键词]博落回;化学成分;生物碱;细胞毒活性
[收稿日期]2014-10-20
[基金项目]国家“重大新药创制”科技重大专项(2011ZX09307-002-01)
[通信作者]*周光雄，Tel： (020)85221469; E-mail： guangxzh@sina.com
[作者简介]邹惠亮， 硕士研究生，E-mail： zouhuiliang@163.comAlkaloids from Macleaya cordata and their cytotoxicity assay
ZOU Hui-liang， LI Hong-yu， YU Shao-fu， CHENG Miao， ZHOU Xing-dong，ZHANG Ying， LIU Bai-lian， ZHOU Guang-xiong*
, 百拇医药
(Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine and
New Drugs Research， Institute of Traditional Chinese Medicine and Natural Products， College of Pharmacy，Jinan University， Guangzhou 510632， China)
[Abstract]Objective： To study the alkaloids of Macleaya cordata and their anti-tumor activities. Method： Alcohol and liquid-liquid extraction were used methods were used to extract the alkaloids constituents， and silica gel， reverse-phase octadecylsilyl(ODS)， sephadex LH-20 chromatographic methods and HPLC were applied to isolate and purify compounds. MS， NMR spectroscopic methods were used to determine their structures. Furthermore， the cytotoxicity of these chemical components for MCF-7 and SF-268 cell lines was measured by MTT method. Result： Twelve alkaloids were isolated from the fruits of M. cordata， and their structures were identified as： maclekarpine E(1)， 6-acetonyldihyrochelerythrine(2)， cavidilinine(3)， 6-acetonyldihyrosanguinnarine (4)， O-methylzanthoxyline(5)， 6-methoxy-dihydrosanguinarine (6)， spallidamine (7)， 6- hydroxyldihydrochelerythrine (8)， arnotianamida (9)， dihydrosanguinarine (10)， protopine (11)， and cryptopine (12). Conclusion： Compounds 1， 3， 7-9 were isolated from M. cordata for the first time， and compound 5 is a new natural product. The results of cytotoxic assay indicated that compound 6 showed strong cytotoxicity against MCF-7 and SF-268 cell lines with IC50 values of 0.61 μmol·L-1 and 0.54 μmol·L-1， respectively., http://www.100md.com(邹惠亮 李红玉 余绍福 程淼 周兴栋 张英 刘百联 周光雄)
, 百拇医药
[关键词]博落回;化学成分;生物碱;细胞毒活性
[收稿日期]2014-10-20
[基金项目]国家“重大新药创制”科技重大专项(2011ZX09307-002-01)
[通信作者]*周光雄，Tel： (020)85221469; E-mail： guangxzh@sina.com
[作者简介]邹惠亮， 硕士研究生，E-mail： zouhuiliang@163.comAlkaloids from Macleaya cordata and their cytotoxicity assay
ZOU Hui-liang， LI Hong-yu， YU Shao-fu， CHENG Miao， ZHOU Xing-dong，ZHANG Ying， LIU Bai-lian， ZHOU Guang-xiong*
, 百拇医药
(Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine and
New Drugs Research， Institute of Traditional Chinese Medicine and Natural Products， College of Pharmacy，Jinan University， Guangzhou 510632， China)
[Abstract]Objective： To study the alkaloids of Macleaya cordata and their anti-tumor activities. Method： Alcohol and liquid-liquid extraction were used methods were used to extract the alkaloids constituents， and silica gel， reverse-phase octadecylsilyl(ODS)， sephadex LH-20 chromatographic methods and HPLC were applied to isolate and purify compounds. MS， NMR spectroscopic methods were used to determine their structures. Furthermore， the cytotoxicity of these chemical components for MCF-7 and SF-268 cell lines was measured by MTT method. Result： Twelve alkaloids were isolated from the fruits of M. cordata， and their structures were identified as： maclekarpine E(1)， 6-acetonyldihyrochelerythrine(2)， cavidilinine(3)， 6-acetonyldihyrosanguinnarine (4)， O-methylzanthoxyline(5)， 6-methoxy-dihydrosanguinarine (6)， spallidamine (7)， 6- hydroxyldihydrochelerythrine (8)， arnotianamida (9)， dihydrosanguinarine (10)， protopine (11)， and cryptopine (12). Conclusion： Compounds 1， 3， 7-9 were isolated from M. cordata for the first time， and compound 5 is a new natural product. The results of cytotoxic assay indicated that compound 6 showed strong cytotoxicity against MCF-7 and SF-268 cell lines with IC50 values of 0.61 μmol·L-1 and 0.54 μmol·L-1， respectively., http://www.100md.com(邹惠亮 李红玉 余绍福 程淼 周兴栋 张英 刘百联 周光雄)