穿心莲内酯对白念珠菌群感效应及相关毒力基因的影响(1)
[摘要]目的：研究穿心莲内酯(andrographolide，AG)对白念珠菌群感效应(quorumsensing，QS)及相关毒力基因的影响。方法：气相色谱-质谱联用仪(GC-MS)检测AG干预白念珠菌不同时间后法尼醇和酪醇的含量变化;qRT-PCR检测QS调节的相关毒力基因CHK1，PBS2，HOG1的表达。结果：白念珠菌生长2h时法尼醇和酪醇分泌量较少，AG作用后变化不明显;12h后分泌量最高，24h回落。不同浓度AG干预后，法尼醇含量降低，酪醇升高，呈现剂量依赖性，其中1000mg·L-1AG效果均最明显。qRT-PCR结果显示，1000mg·L-1AG在2，12，24h时分别使CHK1下调2.375，3.330，4.043倍;PBS2下调2.010，4.210，4.760倍;HOG1变化不明显。结论：AG可抑制法尼醇的分泌及促进酪醇的分泌，下调QS相关的毒力基因CHK1，PBS2的表达。
[关键词]穿心莲内酯;白念珠菌;群感效应;法尼醇;酪醇
, http://www.100md.com Effect of andrographolide on quorum sensing and relevant
virulence genes of Candida albicans
YAN Yuan-yuan1， SHI Gao-xiang1， SHAO Jing1， LU Ke-qiao1， ZHANG Meng-xiang1， WANG Tian-ming1，WANG Bin2， WANG Chang-zhong1*
(1. School of Integrated Traditional and Western Medicine， Anhui University of Chinese Medicine， Integrated Traditional and
, 百拇医药
Western Medicine Research Institution， Anhui Provincial Academy of Chinese Medical Sciences， Hefei 230038， China;
2. Science Experiment Center of Anhui University of Chinese Medicine， Hefei 230038， China)
[Abstract]Objective： To investigate the effect of andrographolide (AG) on quroum sensing (QS) and relevant virulence genes of Candida albicans. Method： Gas-chromatography-mass spectrometry (GC-MS) was applied to detect the changes in the content of farnesol and tyrosol in C. albicans intervened by AG. The real-time quantitative PCR (qRT-PCR) was adopted to inspect the expressions of relevant virulence genes such as CHK1， PBS2 and HOG1 regulated by QS. Result： At 2 h after the growth of C. albican， the farnesol and tyrosol secretions reduced， without notable change after intervention with AG. The secretions were highest at 12 h and decreased at 24 h. After the intervention with different concentrations of AG， the farnesol content reduces， whereas tyrosol increased， indicating a dose-dependence， particularly with 1 000 mg·L-1AG. qRT-PCR revealed that 1 000 mg·L-1 AG could down-regulate CHK1 by 2.375， 3.330 and 4.043 times and PBS2 by 2.010， 4.210 and 4.760 times， with no significant change in HOG1. Conclusion： AG could inhibit the farnesol secretion， promote the tyrosol secretion and down-regulate QS-related virulence genes CHK1 and PBS2 expressions.
[Key words]andrographolide; Candida albicans; quroum sensing; farnesol; tyrosol
doi：10.4268/cjcmm20150224, http://www.100md.com(严园园 施高翔 邵菁 陆克乔 张梦翔 汪天明 王斌 汪长中)
[关键词]穿心莲内酯;白念珠菌;群感效应;法尼醇;酪醇
, http://www.100md.com Effect of andrographolide on quorum sensing and relevant
virulence genes of Candida albicans
YAN Yuan-yuan1， SHI Gao-xiang1， SHAO Jing1， LU Ke-qiao1， ZHANG Meng-xiang1， WANG Tian-ming1，WANG Bin2， WANG Chang-zhong1*
(1. School of Integrated Traditional and Western Medicine， Anhui University of Chinese Medicine， Integrated Traditional and
, 百拇医药
Western Medicine Research Institution， Anhui Provincial Academy of Chinese Medical Sciences， Hefei 230038， China;
2. Science Experiment Center of Anhui University of Chinese Medicine， Hefei 230038， China)
[Abstract]Objective： To investigate the effect of andrographolide (AG) on quroum sensing (QS) and relevant virulence genes of Candida albicans. Method： Gas-chromatography-mass spectrometry (GC-MS) was applied to detect the changes in the content of farnesol and tyrosol in C. albicans intervened by AG. The real-time quantitative PCR (qRT-PCR) was adopted to inspect the expressions of relevant virulence genes such as CHK1， PBS2 and HOG1 regulated by QS. Result： At 2 h after the growth of C. albican， the farnesol and tyrosol secretions reduced， without notable change after intervention with AG. The secretions were highest at 12 h and decreased at 24 h. After the intervention with different concentrations of AG， the farnesol content reduces， whereas tyrosol increased， indicating a dose-dependence， particularly with 1 000 mg·L-1AG. qRT-PCR revealed that 1 000 mg·L-1 AG could down-regulate CHK1 by 2.375， 3.330 and 4.043 times and PBS2 by 2.010， 4.210 and 4.760 times， with no significant change in HOG1. Conclusion： AG could inhibit the farnesol secretion， promote the tyrosol secretion and down-regulate QS-related virulence genes CHK1 and PBS2 expressions.
[Key words]andrographolide; Candida albicans; quroum sensing; farnesol; tyrosol
doi：10.4268/cjcmm20150224, http://www.100md.com(严园园 施高翔 邵菁 陆克乔 张梦翔 汪天明 王斌 汪长中)