四逆汤中甘草与其他药味配伍药效成分变化规律研究(1)
[摘要]研究四逆汤中甘草与其他药味配伍后药效成分甘草苷、甘草酸、甘草素、异甘草素含量的变化。以甘草单煎液、甘草附子合煎液、甘草干姜合煎液、四逆汤为供试品，采用HPLC测定甘草中4种药效成分的含量变化。结果显示甘草配伍不同药味之后4种药效成分含量均降低，且4种药效成分的含量均为甘草单煎液>甘草干姜合煎液>甘草附子合煎液>四逆汤。其中甘草苷质量浓度分别为11.18，9.89，9.67，9.17 mg·g-1;甘草酸质量浓度分别为20.76，15.58，11.30，8.52 mg·g-1;甘草素质量浓度分别为0.66，0.57，0.45，0.24 mg·g-1;异甘草素质量浓度分别为0.14，0.07，0.03，0.01 mg·g-1。因此，甘草配伍干姜后甘草中药效成分含量明显降低，为甘草可缓干姜之烈性提供了科学依据;甘草配伍附子后甘草中药效成分含量大幅度降低，为甘草解附子之毒的物质基础研究提供了科学支撑;甘草与附子、干姜共同配伍后甘草中药效成分含量进一步降低，说明四逆汤方中3味药相互依存相互牵制以共奏药效，体现了四逆汤药味之间相畏/相杀、相须/相使配伍的科学内涵。
, 百拇医药
[关键词]四逆汤;甘草;配伍;含量变化
[收稿日期]2014-09-24
[基金项目]国家自然科学基金项目(81373987)
[通信作者]*傅超美，教授，博士生导师，研究方向为中药新制剂、新工艺和新技术，E-mail：chaomeifu@126.com
[作者简介]彭伟，硕士研究生，E-mail：weipeng0605@126.com
Changing rules study of effective components of Glycyrrhizae Radix et
Rhizoma before and after compatibilities in Sini decoction
, http://www.100md.com
PENG Wei1， JIANG Yan-ping1， FU Chao-mei1*， GAO Fei2， YANG Huan1， LIU Ge-yi1， YU Jia-li1
(1. Pharmacy College， Chengdu University of Traditional Chinese Medicine; Ministry of Education Key Laboratory of
Standardization of Chinese Herbal Medicine， State Key Laboratory Breeding Base of Systematic Research，Development and Utilization of Chinese Medicine Resources， Chengdu 611137， China;
2. Hong Kong University， Li Ka-shing Medical College; Traditional Chinese Medicine College， Hong Kong 999077， China)
, http://www.100md.com
[Abstract]The change of the effective components (liquiritin， glycyrrhizic acid， liquiritigenin， isoliquiritigenin) contents of Glycyrrhizae Radix et Rhizoma (GRR) before and after compatibilities in Sini decoction was studied in this paper. Taking single GRR decoction， GRR-Aconiti Lateralis Radix Praeparata (ALRP) decoction， GRR-Zingiberis Rhizoma (ZR) decoction and Sini decoction as test samples， the contents changing of the four effective components of GRR were measured by HPLC. The results showed that the contents of the four effective components of GRR in the single GRR decoction was higher than that in other samples， and the sequence was single GRR decoction>GRR-ZR decoction>GRR-ALRP decoction>Sini decoction. The contents of liquiritin were 11.18， 9.89， 9.67， 9.17 mg·g-1; the contents of glycyrrhizic acid were 20.76， 15.58， 11.30， 8.52 mg·g-1; the contents of liquiritigenin were 0.66， 0.57， 0.45， 0.24 mg·g-1; the contents of isoliquiritigenin were 0.14，0.07， 0.03， 0.01 mg·g-1. Therefore， the effective components of GRR decreased obviously after GRR compatibility with ZR providing scientific basis for GRR relieving the strong nature of ZR. The effective components of GRR decreased sharply after GRR compatibility with ALRP providing scientific support for the material foundation research of GRR reducing the toxicity of ALRP. The effective components of GRR decreased further in Sini decoction indicating that the three medicines in Sini decoction were interactional， which reflecting the scientific connotation of the mutual-restraint/mutual-detoxication， mutual-promotion/mutual-assistance compatibilities in Sini decoction., 百拇医药(彭伟 蒋燕萍 傅超美 高飞 杨欢 刘哿吚 余佳丽)
, 百拇医药
[关键词]四逆汤;甘草;配伍;含量变化
[收稿日期]2014-09-24
[基金项目]国家自然科学基金项目(81373987)
[通信作者]*傅超美，教授，博士生导师，研究方向为中药新制剂、新工艺和新技术，E-mail：chaomeifu@126.com
[作者简介]彭伟，硕士研究生，E-mail：weipeng0605@126.com
Changing rules study of effective components of Glycyrrhizae Radix et
Rhizoma before and after compatibilities in Sini decoction
, http://www.100md.com
PENG Wei1， JIANG Yan-ping1， FU Chao-mei1*， GAO Fei2， YANG Huan1， LIU Ge-yi1， YU Jia-li1
(1. Pharmacy College， Chengdu University of Traditional Chinese Medicine; Ministry of Education Key Laboratory of
Standardization of Chinese Herbal Medicine， State Key Laboratory Breeding Base of Systematic Research，Development and Utilization of Chinese Medicine Resources， Chengdu 611137， China;
2. Hong Kong University， Li Ka-shing Medical College; Traditional Chinese Medicine College， Hong Kong 999077， China)
, http://www.100md.com
[Abstract]The change of the effective components (liquiritin， glycyrrhizic acid， liquiritigenin， isoliquiritigenin) contents of Glycyrrhizae Radix et Rhizoma (GRR) before and after compatibilities in Sini decoction was studied in this paper. Taking single GRR decoction， GRR-Aconiti Lateralis Radix Praeparata (ALRP) decoction， GRR-Zingiberis Rhizoma (ZR) decoction and Sini decoction as test samples， the contents changing of the four effective components of GRR were measured by HPLC. The results showed that the contents of the four effective components of GRR in the single GRR decoction was higher than that in other samples， and the sequence was single GRR decoction>GRR-ZR decoction>GRR-ALRP decoction>Sini decoction. The contents of liquiritin were 11.18， 9.89， 9.67， 9.17 mg·g-1; the contents of glycyrrhizic acid were 20.76， 15.58， 11.30， 8.52 mg·g-1; the contents of liquiritigenin were 0.66， 0.57， 0.45， 0.24 mg·g-1; the contents of isoliquiritigenin were 0.14，0.07， 0.03， 0.01 mg·g-1. Therefore， the effective components of GRR decreased obviously after GRR compatibility with ZR providing scientific basis for GRR relieving the strong nature of ZR. The effective components of GRR decreased sharply after GRR compatibility with ALRP providing scientific support for the material foundation research of GRR reducing the toxicity of ALRP. The effective components of GRR decreased further in Sini decoction indicating that the three medicines in Sini decoction were interactional， which reflecting the scientific connotation of the mutual-restraint/mutual-detoxication， mutual-promotion/mutual-assistance compatibilities in Sini decoction., 百拇医药(彭伟 蒋燕萍 傅超美 高飞 杨欢 刘哿吚 余佳丽)