脑内同步灌流NaN3致大鼠mPFC和海马细胞外液中胆碱类递质含量变化(4)
[Abstract] Objective： To observe the effect of synchronous perfusion of specific respiratory chain complex IV inhibitor sodium azide (NaN3) in brain on rat ventromedial prefrontal cortex (mPFC) and acetylcholine (ACh) and choline (Ch) contents in hippocampal extra-cellular fluid， and establish the AD rat model induced by mitochondrial acute injury. Method： The synchronous dual-probe dual-channel brain microdialysis sampling technology was applied to synchronously perfuse modified Ringer′s solution containing NaN3 (50 μmol·L-1) and neostigmine (2 μmol·L-1) into mPFC and hippocampus of conscious， freely moving normal rats， and continuously collect dialysates from different encephalic areas. Dynamic contents of ACh and Ch were determined by high performance liquid chromatography-post-column immobilized enzyme reactor-electrochemical process. Result： ACh and Ch contents in mPFC extra-cellular fluid of normal rats were higher than that in hippocampus. During the process of perfusion， NaN3 could significantly reduce ACh in mPFC/hippocampal extra-cellular fluid， but remarkably increase Ch， and constantly inhibit the recovery of ACh and Ch contents in mPFC/hippocampus. Conclusion： The synchronous perfusion of NaN3 in rat mPFC and hippocampus can injure functions of the cholinergic nerve projection area， and cause the acute AD model with ACh and Ch metabolic disorders. This model can be used in pathogenetic and pharmacological studies.
[Key words] synchronous dual-probe brain microdialysis； sodium azide； acetylcholine； choline； ventromedial prefrontal cortex； hippocampus
doi：10.4268/cjcmm20140324
[责任编辑 张宁宁], http://www.100md.com(张美玉 孙丹丹 刘洋 崔悦 赵小亮 张莹 王志国 王丹巧)
[Key words] synchronous dual-probe brain microdialysis； sodium azide； acetylcholine； choline； ventromedial prefrontal cortex； hippocampus
doi：10.4268/cjcmm20140324
[责任编辑 张宁宁], http://www.100md.com(张美玉 孙丹丹 刘洋 崔悦 赵小亮 张莹 王志国 王丹巧)