泰山赤灵芝复合降解酶提取白花丹参须根有效成分的研究(4)
(Department of Biological Sciences， Taishan Medical College， Taian 271016， China)
[Abstract]Objective： To study the application of degrading multi-enzymes from Ganoderma lucidum in extracting effective constituents from fibrous roots of Salvia miltiorrhiza. Method： Effective constituents were extracted from fibrous roots by degrading multi-enzymes of wood fiber. The enzymatic parameters were optimized by the orthogonal design. Result： The extraction efficiencies of total tanshinones and total salvianolic acids in the extracts of fibrous roots of S. miltiorrhiza was obtained using optimum enzymolysis process reached 11.923%， 12.465%， respectively， which were 62.794%， 56.086% more than that by conventional non-enzymatic hydrolysis. Conclusion： Degrading multi-enzymes of wood fiber can be used to fully extract effective constituents from fibrous roots of S. miltiorrhiza， which provides a new approach for recycling wastes of traditional Chinese medicines.
[Key words]Ganoderma lucidum； laccase； cellulase； Salvia miltiorrhiza； effective component
doi：10.4268/cjcmm20130512[责任编辑曹阳阳], http://www.100md.com(李艳玲 辛晓明 苗增民 史仁玖 郝岗平)
[Abstract]Objective： To study the application of degrading multi-enzymes from Ganoderma lucidum in extracting effective constituents from fibrous roots of Salvia miltiorrhiza. Method： Effective constituents were extracted from fibrous roots by degrading multi-enzymes of wood fiber. The enzymatic parameters were optimized by the orthogonal design. Result： The extraction efficiencies of total tanshinones and total salvianolic acids in the extracts of fibrous roots of S. miltiorrhiza was obtained using optimum enzymolysis process reached 11.923%， 12.465%， respectively， which were 62.794%， 56.086% more than that by conventional non-enzymatic hydrolysis. Conclusion： Degrading multi-enzymes of wood fiber can be used to fully extract effective constituents from fibrous roots of S. miltiorrhiza， which provides a new approach for recycling wastes of traditional Chinese medicines.
[Key words]Ganoderma lucidum； laccase； cellulase； Salvia miltiorrhiza； effective component
doi：10.4268/cjcmm20130512[责任编辑曹阳阳], http://www.100md.com(李艳玲 辛晓明 苗增民 史仁玖 郝岗平)