n快速识别与鉴定> 清火栀麦片中化学成分的SPE—HPLC—ESI—MSn快速识别与鉴定(5)
Rapid recognition and identification of chemical constituents in
Qinghuo Zhimai tablets by SPE-HPLC-ESI-MSn
ZHANG Jia-yu1，2， QIAO Yan-jiang1*， GAO Xiao-yan2， ZHANG Qian1， MA Qun1， LU Jian-qiu2*
(1.School of Chinese Pharmacy， Beijing University of Chinese Medicine， Beijing 100102， China；
2.Center of Scientific Experiment， Beijing University of Chinese Medicine， Beijing 100029， China)
[Abstract] Objective： To establish a method for analyzing solid phase extraction-high performance liquid chromatography-electrospray ionization tandem mass spectrometry (SPE-HPLC-ESI-MSn)， in order to recognize and identify the main chemical constituents in Qinghuo Zhimai tablets.Method： The possible structures of the compounds were determined according to the structure information of compounds observed from molecular ion peaks and fragment ions in HPLC-ESI-MSn negative ion mode and by comparing with literature data or control samples.Result： Through the comparative analysis on Qinghuo Zhimai Tablets and components of its formula， 39 chemical constituents were identified， including 7 caffeoylquinic acids， 7 iridoids， 6 diterpenoid lactones， 5 homoisoflavonoids， 13 steroidal saponins and 1 flavone glycoside.Conclusion： This study provides a simple and rapid method for identifying chemical components in Qinghuo Zhimai tablets.
[Key words] HPLC-ESI-MSn； SPE； Qinghuo Zhimai tablets； identification of chemical constituents
doi：10.4268/cjcmm20130209
[责任编辑 曹阳阳], 百拇医药(张加余,乔延江,高晓燕,张倩,马群,卢建秋)
Qinghuo Zhimai tablets by SPE-HPLC-ESI-MSn
ZHANG Jia-yu1，2， QIAO Yan-jiang1*， GAO Xiao-yan2， ZHANG Qian1， MA Qun1， LU Jian-qiu2*
(1.School of Chinese Pharmacy， Beijing University of Chinese Medicine， Beijing 100102， China；
2.Center of Scientific Experiment， Beijing University of Chinese Medicine， Beijing 100029， China)
[Abstract] Objective： To establish a method for analyzing solid phase extraction-high performance liquid chromatography-electrospray ionization tandem mass spectrometry (SPE-HPLC-ESI-MSn)， in order to recognize and identify the main chemical constituents in Qinghuo Zhimai tablets.Method： The possible structures of the compounds were determined according to the structure information of compounds observed from molecular ion peaks and fragment ions in HPLC-ESI-MSn negative ion mode and by comparing with literature data or control samples.Result： Through the comparative analysis on Qinghuo Zhimai Tablets and components of its formula， 39 chemical constituents were identified， including 7 caffeoylquinic acids， 7 iridoids， 6 diterpenoid lactones， 5 homoisoflavonoids， 13 steroidal saponins and 1 flavone glycoside.Conclusion： This study provides a simple and rapid method for identifying chemical components in Qinghuo Zhimai tablets.
[Key words] HPLC-ESI-MSn； SPE； Qinghuo Zhimai tablets； identification of chemical constituents
doi：10.4268/cjcmm20130209
[责任编辑 曹阳阳], 百拇医药(张加余,乔延江,高晓燕,张倩,马群,卢建秋)